84-5185-21　DAAM1 100ul　522142

特徴

• Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation.
• May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation.
• Can direct nucleation and elongation of new actin filaments.
• Involved in building functional cilia (PubMed:16630611, PubMed:17482208).
• Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity).
• Together with DAAM2, required for myocardial maturation and sarcomere assembly (By similarity)
• after thawing and prior to removing the cap.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.