84-5180-97　CTNND1 200ul　521926

特徴

• Catenin delta-1 (p120 catenin) has an N-terminal coiled-coil domain followed by a regulatory domain containing multiple phosphorylation sites and a central Armadillo repeat domain of ten linked 4aa repeats.
• The C-terminal tail has no known function (1).
• Catenin delta-1 fulfills critical roles in the regulation of cell-cell adhesion as it regulates E- cadherin turnover at the cell surface to determine the level of E-cadherin available for cell-cell adhesion (2).
• Catenin delta-1 has both positive and negative effects on cadherin-mediated adhesion (3).
• Actin dynamics are also regulated by catenin delta-1, which modulates RhoA, Rac, and cdc42 proteins (1).
• Analogous to beta-catenin, catenin delta-1 translocates to the nucleus, although its role at this location is unclear.
• Many studies show that catenin delta-1 is expressed irregularly or is absent in various types of tumor cells, suggesting that catenin delta-1 may function as a tumor suppressor (4).
• Phosphorylation of Tyr904 on Catenin-delta-1 was identified at Cell Signaling Technology (CST) using PhosphoScan, a CST's LC-MS/MS platform for phosphorylation site discovery (5).
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.