84-5174-39　CLASP2 200ul　521585

特徴

• Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules (PubMed:26003921).
• Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN).
• Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell.
• May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2 (PubMed:16824950).
• This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling.
• Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (PubMed:16866869, PubMed:16914514).
• Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:3000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
• ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.