84-5172-30　CHD8 100ul　521476

特徴

• DNA helicase that acts as a chromatin remodeling factor and regulates transcription.
• Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes.
• Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity.
• Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity.
• Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes.
• Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF.
• Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity.
• Also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:3000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.