84-5172-21　CHD1 200ul　521470

特徴

• ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA.
• Regulates polymerase II transcription.
• Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step.
• Regulates negatively DNA replication.
• Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome.
• Is also associated with histone deacetylase (HDAC) activity (By similarity).
• Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3.
• Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3 (PubMed:18042460, PubMed:28866611).
• Required for maintaining open chromatin and pluripotency in embryonic stem cells (By similarity).
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:3000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
• ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.