Cholesteryl ester transfer protein (CETP) is a circulating metabolic enzyme that transports cholesterol from the arteries to the liver (1-3).
CETP converts cholesteryl esters from antiatherogenic high density lipoproteins (HDLs) to proatherogenicapolipoprotein B (apoB)-containing lipoproteins, including very low-, intermediate-, and low-DLs (3).
Efficient transfer and exchange of cholesteryl esters and triglycerides between the lipoprotein classes of human plasma is necessary to maintain healthy arteries (4,5).
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography from serum.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes endogenous levels of total human CETP. Species Crossreactivity: Mouse
Isotype:IgG
Calc Applications Abbrev:E IC IF IHC WB
Calc Crossreactivity:Hu Mo
Immunogen:Synthetic peptide corresponding to human CETP.