DNA-binding component of the FA core complex involved in DNA damage repair and genome maintenance.
Required for optimal chromatin association of the FA core complex.
Required for efficient damage-induced monoubiquitination and focus formation of FANCD2.
Stabilizes FAAD24, FANCM and STRA13/CENPX in the FA core complex.
Plays a role in DNA interstrand cross-linking (ICL) repair and in recorery of replication forks stalled by topoisomerase I-DNA cleavage intermediates induced by camptothecin.
As a component of the APITD1/CENPS complex, is also essential for the stable assembly of the outer kinetchore.
Component of the CENPA- CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight.
Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total CENPS. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to CENPS at C-terminal.
