84-5170-89　CENPF 200ul　521404

特徴

• Required for kinetochore function and chromosome segregation in mitosis.
• Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1.
• Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25.
• Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation.
• May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes.
• Interaction with RB directs embryonic stem cells toward a cardiac lineage.
• Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus.
• Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis.
• Involved in dendritic cell regulation of T-cell immunity against chlamydia.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight.
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.