84-5168-97　CDCA8 200ul　521299

特徴

• Borealin is a 280aa protein that stabilizes the bipolar mitotic spindle.
• Borealin is part of a complex called Aurora B kinase that has essential regulatory roles at centromeres and the central spindle in cells undergoing mitosis.
• This complex makes up the enzymatic core of the chromosomal passenger complex (CPC).
• It is possible that the Borealin subunit directs the CPC to centromeric DNA.
• Deletion of Borealin delays mitotic progression and causes kinetochore-spindle to mis-attach.
• Deletion also causes an increase in bipolar spindles associated with ectopic asters.
• These extra poles, which form after chromosomes acquire their bipolar orientation, seriously disrupt chromosome allotment during anaphase.
• It is true that inappropriate chromosomal segregation and cell division may cause auneuploidy leading to cancer, so an abnormal expression of Borealin may lead to gastric cancer.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.