Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression.
Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner.
Directly dephosphorylates CDK1 and stimulates its kinase activity.
The three isoforms seem to have a different level of activity.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Phospho-CDC25B(Ser151).
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthesized peptide derived from human CDC25B around the phosphorylation site of Ser151. Species sequence homology: Porcine (83%), Bovine (88%), Equine (92%), Sheep (83%), Rabbit (92%), Canine (92%)
