This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non- major histocompatibility complex (MHC) restricted killing.
The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity.
Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:2000, incubate membrane with TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200.
Heat antgen retrieval with citrate buffer is recommended
Immunofluorescence (IF-IC): 1:100-1:500ELISA (Peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol
Specificity:Recognizes endogenous levels of total human CD244. Species Crossreactivity: mouse and rat
Isotype:IgG
Calc Applications Abbrev:E IC IF IHC WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Synthetic peptide corresponding to a region within N-terminal amino acids of human CD244