84-5165-08　CCR5, phosphorylated (Ser349) 200ul　521073

特徴

• CCR5 a 7-transmembrane G-linked receptor for a number of inflammatory C-C type chemokines including MIP-1-alpha, MIP-1-beta and RANTES.
• Transduces a signal by increasing the intracellular calcium ion level.
• May play a role in the control of granulocytic lineage proliferation or differentiation.
• Acts as a coreceptor (along with CD4) for HIV-1 R5 isolates.
• Interacts with PRAF2.
• Interacts with HIV-1 surface protein gp120.
• Efficient ligand binding to CCL3/MIP-1alpha and CCR4/MIP-1beta requires sulfation, O-glycosylation and sialic acid modifications.
• Glycosylation on S6 is required for efficient binding of CCL4.
• Applications:Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.