84-5164-52　CCL5 100ul　521043

特徴

• This gene is one of several chemokine genes clustered on the q-arm of chromosome 17.
• Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes.
• The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide.
• This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils.
• It causes the release of histamine from basophils and activates eosinophils.
• This cytokine is one of the major HIV suppressive factors produced by CD8+ cells.
• It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor.
• Alternative splicing results in multiple transcript variants that encode different isoforms.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.