Chemokines are members of a superfamily of small inducible, secreted, pro-inflammatory cytokines.
Members of the chemokine family exhibit 20 to 50% homology in their predicted amino acid sequences and are divided into four subfamilies.
In C-C (or b) subfamily, the first two cysteines are adjacent.
C-C chemokines are chemoattractants and activators for monocytes and T cells.
C-C subfamily members include macrophage inflammatory protein (MIP)-1 , MIP-1∫, MIP-2, MIP-3 , MIP-3∫, MIP-4, HCC-1, MIP-5 (or HCC-2), RANTES, MCP-1/2/3 (and the murine homologs JE and MARC), I-309, murine C10 and TCA3.
Research has shown that MIP-1∫ is more selective than MIP-1 , primarily attracting CD4+ T lymphocytes, with a preference for T cells of the naive phenotype.
MIP-1 is a more potent lymphocyte chemoattractant than MIP-1∫ and exhibits a broader range of chemoattractant specificities.
It has been suggested that CD8+ T lymphocytes are involved in the control of HIV infection in vivo by the release of HIV-suppressive factors (HIV-SF).
MIP-1 has been identified as one of the major HIV- SFs produced by CD8+ T cells, along with MIP-1∫ and RANTES.
Recombinant human MIP-1 acts as an inhibitor of different strains of HIV-1, HIV-2 and SIV infection in a dose- dependent manner.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total CCL4. Species Crossreactivity: Mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human CCL4. Species sequence homology: Porcine (100%), Bovine (91%), Equine (100%), Rabbit (82%), Canine (91%)
