Non-apoptotic caspase involved in epidermal differentiation.
Is the predominant caspase in epidermal stratum corneum(PubMed:15556625).
Seems to play a role in keratinocyte differentiation and is required for cornification.
Regulates maturation of the epidermis by proteolytically processing filaggrin (By similarity).
In vitro has a preference for the substrate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF (PubMed:16854378, PubMed:19960512).
Involved in processing of prosaposin in the epidermis (By similarity).
May be involved in retinal pigment epithelium cell barrier function (PubMed:25121097).
Involved in DNA degradation in differentiated keratinocytes probably by cleaving DFFA/ICAD leading to liberation of DFFB/CAD (PubMed:24743736).
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human Caspase-14.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human Caspase-14. Species sequence homology: porcine (80%), bovine (90%), equine (100%), sheep (90%), rabbit (80%), canine (100%)
