Involved in the activation cascade of caspases responsible for apoptosis execution.
Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner.
May participate in the granzyme B apoptotic pathways.
Cleaves and activates caspase- 3, -4, -6, -7, -8, and -9.
Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC.
Belongs to the peptidase C14A family.
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17kD (p23/17) (depending on the splicing events) and a 12kD (p12) subunit.
Self-associates.
Interacts with FADD and CASP8.
Found in a Fas signaling complex consisting of FAS, FADD, CASP8 and CASP10.
6 isoforms of the human protein are produced by alternative splicing.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:3000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200Immunofluorescence/Immunocytochemistry (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human Caspase 10. Species Crossreactivity: Mouse
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human Caspase 10.
