Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1).
Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments.
Cleavage of caspase-3 requires aspartic acid at the P1 position (2).
Applications:Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry, Immunoprecipitation, ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:50-1:100, Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total CASP3. Species Crossreactivity: Human, mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IC IF IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human CASP3.
