84-5162-22　Casein Kinase Igamma2 100ul　520926

特徴

• Serine/threonine-protein kinase.
• Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates.
• It can phosphorylate a large number of proteins.
• Participates in Wnt signaling.
• Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3.
• Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles.
• Regulates fast synaptic transmission mediated by glutamate.
• SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses.
• Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis.
• Triggers PER1 proteasomal degradation probably through phosphorylation.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Immunohistochemistry :1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.