Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum.
It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits.
It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.
Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation.
Additionally it may play a role in receptor-mediated endocytosis at the synapse.
Applications:Suitable for use in Western Blot and Immunohistochemistry, ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Phospho-Calnexin(Ser564). Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthesized peptide derived from human Calnexin around the phosphorylation site of Ser564. Species sequence homology: Porcine (100%), Bovine (83%), Equine (83%), Sheep (92%), Rabbit (100%), Canine (83%)
