84-5159-73　CA2 100ul　520798

特徴

• Carbonic anhydrases (CA) are a family of ancient zinc metalloenzymes found in almost all living organisms.
• All CA can be divided into 3 distinct classes (alpha, beta, and gamma) that evolved independently and have no significant homology in sequence and overall folding.
• All functional CA catalyze the reversible hydration of CO2 into HCO3- and H+ and contain a zinc atom in the active sites essential for catalysis.
• There are many isoforms of CA in mammals and they all belong to the alpha class (1,2).
• CA2 is a cytosolic member of the alpha class.
• It is the most widely distributed isoform among the mammalian CAs (1).
• Defects in CA2 are associated with osteopetrosis and renal tubular acidosis (3-5).
• Elevated expression of CA2 is observed in patients with Alzheimer’s disease and the developing brains of Down syndrome patients (6,7).
• CA2 is also overexpressed in Gastrointestinal Stromal Tumors (GISTs) and is considered a useful marker for diagnosis (8).
• Recently, CA2 was reported to facilitate transporter activity of the monocarboxylate transporter isoform 1 and 4 (MCT1/4) independent of its own catalytic activity (9,10)
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.