84-5158-13　c-Cbl, phosphorylated (Tyr700) 100ul　520715

特徴

• Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors.
• Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome.
• Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling.
• Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation.
• Participates in signal transduction in hematopoietic cells.
• Plays an important role in the regulation of osteoblast differentiation and apoptosis.
• Essential for osteoclastic bone resorption.
• The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function.
• May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.