The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle.
One of its checkpoint functions is to inhibit the activity of the anaphase-promotingcomplex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity.
The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE.
Required for kinetochore localization of CENPE.
Negatively regulates PLK1 activity ininterphase cells and suppresses centrosome amplification.
Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest.
May play a role for tumor suppression.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary Ab in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human BUB1B. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human BUB1B. Species sequence homology: porcine (%), bovine (%), equine (%), sheep (%), rabbit (%), canine (%)
