84-5157-79　BTK, phosphorylated (Tyr551) 200ul　520697

特徴

• Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling.
• Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation.
• After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members.
• PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK.
• BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways.
• Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway.
• The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense.
• Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells.
• Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation.
• BTK plays also a critical role in transcription regulation.
• Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes.
• BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B.
• Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR.
• GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression.
• ARID3A and NFAT are other transcriptional target of BTK.
• BTK is required for the formation of functional ARID3A DNA-binding complexes.
• There is however no evidence that BTK itself binds directly to DNA.
• BTK has a dual role in the regulation of apoptosis.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.