84-5157-25　BRD7 200ul　520669

特徴

• Acts both as coactivator and as corepressor.
• May play a role in chromatin remodeling.
• Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity.
• Induces dephosphorylation of GSK3B at 'Tyr-216'.
• Down-regulates TRIM24-mediated activation of transcriptional activation by AR (By similarity).
• Transcriptional corepressor that down-regulates the expression of target genes.
• Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac).
• Binds to the ESR1 promoter.
• Recruits BRCA1 and POU2F1 to the ESR1 promoter.
• Coactivator for TP53-mediated activation of transcription of a set of target genes.
• Required for TP53-mediated cell-cycle arrest in response to oncogene activation.
• Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters.
• Inhibits cell cycle progression from G1 to S phase.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.