84-5157-13　BRCA1, phosphorylated (Ser1457) 200ul　520663

特徴

• This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor.
• The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC for BRCA1-associated genome surveillance complex.
• This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complex.
• This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination.
• Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers.
• Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene.
• Many alternatively spliced transcript variants have been described for this gene but only some have had their full-length natures identified.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.