84-5157-01　BRAF 200ul　520657

特徴

• BRAF: v-raf murine sarcoma viral oncogene homolog B1, also known as BRAF1; RAFB1; B-RAF1; FLJ95109.
• Entrez Protein NP_004324.
• It is the main effectors recruited by GTP- bound Ras to activate the MEK-MAP kinase pathway.
• B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439).
• B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long N-terminal region, the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c- Raf at the plasma membrane, followed by phosphorylation of Thr118 in the N-terminal B-Raf-specific region.
• Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP Ras.
• Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers.
• The BRAF gene mutation is frequently detected in papillary thyroid carcinoma, melanocytic nevi, primary cutaneous melanomas and colorectal cancers.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.