Bcl10/CIPER/CLAP/mE10 is a widely expressed CARD (caspase recruitment domain) containing protein shown to induce apoptosis and activate NF-kappa B (1-5).
The CARD domain mediates self-oligomerization, interactions with other CARD proteins and is necessary for NF-kappa B activation, although the precise mechanism which Bcl10 regulates these processes is not fully understood.
The discovery of Bcl10 came from observations of the chromosomal translocation t(1;14)(p22;q32) from B cell lymphomas of the mucosa-associated lymphoid tissue (MALT) (1,5).
This translocation results in deregulated expression of a truncated form of Bcl10 which lacks apoptotic activity and enhances transformation.
Studies from Bcl10 deficient mice demonstrate that Bcl10 is essential for the activation of NF- kappa B by T- and B-cell receptors (6).
One third of Bcl10 deficient mice developed lethal exencephaly.
Surviving mice were unaffected by various apoptotic stimuli, but were severely immunodeficient and defective in antigen receptor-induced NF-kappa B activiation.
PKC or T-cell receptor signaling results in a downregulation of Bcl10 protein levels, attenuating both NF-kappa B activation and cellular proliferation and also provides a negative feedback regulation of the NF-kappa B signaling to T cell signaling (7).
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human BCL10. Species Crossreactivity: Mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human BCL10.
