84-5154-33　Bax 100ul　520508

特徴

• Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein.
• Induces the release of cytochrome c, activation of CASP3, and thereby apoptosis.
• Belongs to the Bcl-2 family.
• Homodimer.
• Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), MCL1 and A1.
• Interacts with SH3GLB1 and HN.
• Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm.
• Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria.
• Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L).
• 8 isoforms of the human protein are produced by alternative splicing.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:3000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween-20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.