84-5150-71 ATF1, phosphorylated (Ser63) 100ul 520322
特徴
- ATF-1 a transcription factor that is a member of the leucine zipper family.
- Forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription.
- Binds the Tax-responsive element (TRE) of HTLV-I.
- Activated downstream of IL-1.
- c-Src and TRAF6 are mediators of IL-1-induced AP-1 activation.
- Applications:Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and ELISA.
- Other applications not tested.
- Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
- Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
- Storage and Stability:May be stored at 4°C for short-term only.
- Aliquot to avoid repeated freezing and thawing.
- Store at -20°C.
- Aliquots are stable for 12 months after receipt.
- For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
- Size:100ul
- Host:rabbit
- Source Antibody:human
- Grade:Affinity Purified
- Purity:Purified by immunoaffinity chromatography from serum.
- Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Specificity:Recognizes endogenous levels of human Phospho-ATF1 (Ser63). Species Crossreactivity: Mouse
- Isotype:IgG
- Calc Applications Abbrev:E IC IF WB
- Calc Crossreactivity:Hu Mo
- Immunogen:Synthetic peptide corresponding to human ATF1 around the phosphorylation site of Serine 63.
- Swiss Prot Number:P18846
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