84-5149-69　ARRB2 200ul　520270

特徴

• Arrestin proteins function as negative regulators of G protein- coupled receptor (GPCR) signaling.
• Cognate ligand binding stimulates GPCR phosphorylation, which is followed by binding of arrestin to the phosphorylated GPCR and the eventual internalization of the receptor and desensitization of GPCR signaling (1).
• Four distinct mammalian arrestin proteins are known.
• Arrestin 1 (also known as S-arrestin) and arrestin 4 (X-arrestin) are localized to retinal rods and cones, respectively.
• Arrestin 2 (also known as beta-arrestin 1) and arrestin 3 (beta-arrestin 2) are ubiquitously expressed and bind to most GPCRs (2).
• beta-arrestins function as adaptor and scaffold proteins and play important roles in other processes, such as recruiting c-Src family proteins to GPCRs in Erk activation pathways (3,4).
• beta-arrestins are also involved in some receptor tyrosine kinase signaling pathways (5-8).
• Additional evidence suggests that beta-arrestins translocate to the nucleus and help regulate transcription by binding transcriptional cofactors (9,10).
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.