84-5147-27　APEX1 200ul　520139

特徴

• Mitochondrion.
• The cleaved APEX2 is only detected in mitochondria (By similarity).
• Translocation from the cytoplasm to the mitochondria is mediated by ROS signaling and cleavage mediated by granzyme A.
• Tom20-dependent translocated mitochondrial APEX1 level is significantly increased after genotoxic stress and Nucleus.
• Nucleus, nucleolus.
• Nucleus speckle.
• Endoplasmic reticulum.
• Cytoplasm.
• Detected in the cytoplasm of B-cells stimulated to switch (By similarity).
• Colocalized with SIRT1 in the nucleus.
• Colocalized with YBX1 in nuclear speckles after genotoxic stress.
• Together with OGG1 is recruited to nuclear speckles in UVA-irradiated cells.
• Colocalized with nucleolin and NPM1 in the nucleolus.
• Its nucleolar localization is cell cycle dependent and requires active rRNA transcription.
• Colocalized with calreticulin in the endoplasmic reticulum.
• Translocation from the nucleus to the cytoplasm is stimulated in presence of nitric oxide (NO) and function in a CRM1-dependent manner, possibly as a consequence of demasking a nuclear export signal (aa64-80).
• S-nitrosylation at Cys-93 and Cys-310 regulates its nuclear-cytosolic shuttling.
• Ubiquitinated form is localized predominantly in the cytoplasm.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200, Immunofluorescence: 1:100-400 ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.