84-5146-73　ANXA5 100ul　520113

特徴

• Annexin V, also known as PAP-1 or Lipocortin V, is a ~30kD protein that binds to phospholipids in a calcium-dependent manner (1).
• All annexins contain a putative PKC binding site, but only annexin V has been identified as an inhibitor of this pathway (2).
• It may also signal, by direct interaction with VEGFR2 receptor, in the regulation of vascular endothelial cell proliferation (3).
• Annexin V preferentially binds phosphatidylserine, in competition with prothrombin, leading to inhibition of blood coagulation at sites of injury preceding contact between lipid components and coagulation factors that initiate thrombosis (4-6).
• The ability of Annexin V to bind specifically and robustly to hosphatidylserine makes it an attractive reagent in detecting apoptotic cells (7).
• Annexin V is inducible by glucocorticoids and can be phosphorylated by tyrosine and serine/threonine kinases (8).
• It isthought to blockproduction of mediators of inflammation, such as prostaglandins and leukotrienes by inhibiting the release of arachidonic acid from membranes by phospholipase A2 (8).
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.