84-5146-59　ANXA1 100ul　520106

特徴

• The annexin superfamily consists of 13 calcium or calcium and phospholipid binding proteins with high biological and structural homology (1).
• Annexin-1 (ANXA1) is the first characterized member of the annexin family of proteins and is able to bind to cellular membranes in a calcium-dependent manner, promoting membrane fusion and endocytosis (2-4).
• Annexin A1 has anti-inflammatory properties and inhibits phospholipase A2 activity (5,6).
• Annexin A1 can accumulate on internalized vesicles after EGF-stimulated endocytosis and may be required for a late stage in inward vesiculation (7).
• Phosphorylation by PKC, EGFR, and Chak1 results in inhibition of annexin A1 function (8-10).
• Annexin A1 has also been identified as one of the 'eat- me' signals on apoptotic cells that are to be recognized and ingested by phagocytes (11).
• Annexin A1, as an endogenous anti-inflammatory mediator, has roles in many diverse cellular functions, such as membrane aggregation, inflammation, phagocytosis, proliferation, apoptosis, and tumorigenesis and cancer development (12-14).
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.