84-5144-97　Alpha Adaptin 100ul　520022

特徴

• Component of the adaptor protein complex 2 (AP-2).
• Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways.
• Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation.
• AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome.
• The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components.
• Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation.
• AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis.
• AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface.
• AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules.
• AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway.
• The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane.
• The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins.
• The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity).
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.