84-5144-33　AKT3 100ul　519986

特徴

• Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity).
• General protein kinasecapable of phosphorylating several known proteins.
• Phosphorylates TBC1D4.
• Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin andinsulin-like growth factor I (IGF-I).
• Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface.
• Mediates the antiapoptotic effects of IGF-I.
• Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leadingto both phosphorylation of 4E-BP1 and in activation of RPS6KB1.
• Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
• The activated form can suppress FoxO gene transcription and promote cell cycle progression.
• Essential for theSPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary Ab in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.