84-5142-48　AFP 200ul　519888

特徴

• Alpha-fetoprotein (AFP) is a 65kD glycoprotein found in the serum of the mammalian fetal liver, yolk sac, and GI tract.
• While AFP expression in adult cells is low, it is aberrantly expressed in adult liver cancer cells (1,2).
• The tumor suppressor gene p53 and beta-catenin are both involved in the regulation of AFP expression.
• In normal adult cells, p53 binds to the repressor region of the AFP gene, thereby blocking transcription.
• Mutations in both p53 and beta-catenin are associated with aberrant expression of AFP.
• Elevated serum levels are predictive of hepatocellular carcinoma (3).
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:10-1:100ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.