Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress.
Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'.
Activity suppressed by insulin.
Main regulator of redox balance and osteoblast numbers and controls bone mass.
Orchestrates the endocrine function of the skeleton in regulating glucose metabolism.
Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity.
Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP.
In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC and PCK1.
Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and STK4/MST1.
Promotes neural cell death.
Mediates insulin action on adipose tissue.
Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake.
Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells.
Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner.
Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling (By similarity).
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human AC-FKHR(K294). Species Crossreactivity: mouse, rat and monkey
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthesized peptide derived from human AC-FKHR(K294). Species sequence homology: Porcine (100%), Bovine (100%), Canine (100%), Chicken (100%), Xenopus (91%)
