Chemiluminescence systems have emerged as the best all-around method for western blot detection.
They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates.
The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently.
Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies.
HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal.
HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.
Applications:Suitable for use in Western Blot.
Other applications not tested
Recommended Dilutions:Optimal dilutions to be determined by researcher.
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from monospecific antiserum using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP). Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins.
