84-5136-64　［取扱停止］Surface Lipoprotein p27, Borrelia burgdorferi 25ug　516614

特徴

• The p27 gene is located on a linear plasmid of a size of approximately 55kb and encodes a basic protein of 248aa with a typical prokaryotic leader sequence of 17aa residues at the N-terminal of the proposed translation product, as confirmed by Northern and Western blot analysis (Reindl, et al., 1993).
• Spirochetal outer surface proteins (Osps) play a major role in both protection from and pathogenesis of Lyme disease.
• All Osps (OspA to OspF and P27) have been shown to be lipoproteins, containing hydrophobic N-terminal domains and putative cleavage sites (Leu-X-Y-Z-Cys) thought to be recognized by a lipoprotein signal peptidase (Brandt, et al., 1990).
• Borrelia spirochetes are unique among gram-negative (diderm or double-membrane) bacteria in their abundance of surface-displayed lipoproteins, some of which play important roles in the pathogenesis of Lyme disease and relapsing fever.
• There is evidence that Borrelia lipoproteins are specifically targeted to the bacterial surface, but that they can be retained in the periplasm by sequence-specific signals (Schulze & Zückert, 2006).
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1000Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.