BmpA belongs to the BMP lipoprotein family and is involved in borrelial pathogenicity and participates in the development of borrelial arthritis.
It is a member of the chromosomally-located paralogous family 36 which also includes BmpB, BmpC and BmpD.
Its expression is co-regulated with that of BmpC and BmpB and may be subject to global regulation (Bryksin, et al., 2010).
The spirochete migrates from the tick mid-gut during feeding to its salivary glands and is then transmitted to the mammal host.
This transition may be facilitated by changes in expression of some B.
burgdorferi genes (Aron, et al., 1996).
It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host.
The major products of the B.
burgdorferi basic membrane protein (bmp) A/B operon that are induced in murine and human joints possess inflammatory properties.
BmpA and its three paralogous proteins, BmpB, C and D, all bind to mammalian laminin and BmpA-directed antibodies, significantly inhibiting the adherence of live B.
burgdorferi to laminin.
The laminin-binding domain of BmpA was mapped to the 80 C-terminal residues.
Solubilized collagen inhibited BmpA-laminin binding, suggesting interactions through the collagen-binding domains of laminin.
BmpA does not bind mammalian type I or type IV collagens or fibronectin (Verma, et al., 2009).
BmpA is expressed during the invasion of the spirochete and in the evolution of the arthritis of Lyme disease in mammals.
Non-lipidated and lipidated versions of BmpA have been shown to induce the pro-inflammatory cytokines TNF-alpha and IL-1beta in human synovial cells.
The induction of cytokine responses in synovial cells via activation of the NF-kappaB and p38 MAP kinase pathways could potentially contribute to the pathogenesis of Lyme arthritis (Yang, et al., 2008).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes Borrelia burgdorferi P39, Strain B31 (ATCC 35210), derived by limited dilutional cloning from the original Lyme-disease tick isolate obtained by A. Barbour (Johnson, et al., 1984). BLAST analysis suggest cross-reactivity with p39 from B. burgdorferi and B. afzelii sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against B. garinii sources based on 88-95% homology and to B. spielmanii A14S based on 86-96% homology. Crossreactivity with p39 from other sources has not been determined.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Recombinant protein corresponding to B. burgdorferi P39, fused to MBP-Tag.
