The bba64 gene of Borrelia burgdorferi encodes a surface-exposed lipoprotein, called P35.
The 35kD antigen has been shown to be a statistically significant marker in IgG immunoblots in a study of patients with early Lyme disease who presented with erythema migrans (Fikrig, et al., 1997).
P35 expression is induced in the tick vector during feeding and maintained during infection of the vertebrate host.
The expression of a majority of the proteins expressed in early Lyme disease is affected by pH, being abundantly expressed at pH 7.0 (resembling the tick mid-gut pH of 6.8 during feeding) but only sparsely at pH 8.0 (a condition closer to that of the unfed tick mid-gut pH of 7.4) and the encoding genes may be coregulated (Gilmore Jr, et al., 2010).
Borrelia may also change its antigenic composition in its need for adaptation to stresses imposed by changes in conditions from cycling between its arthropod and mammalian hosts (Fikrig, et al., 1997).
The expression of bba64 is down-regulated in the absence of RpoS which has also been shown to be the sole determinant of differential bba64 expression in cultured spirochetes.
P35 expression in B.
burgdorferi is upregulated in the stationary growth phase, and influenced by a temperature of 34°C (but not 24°C) (Gautam, et al., 2008).
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：25ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes Borrelia burgdorferi P35, Strain B31 (ATCC 35210), derived by limited dilutional cloning from the original Lyme-disease tick isolate obtained by A. Barbour (Johnson, et al., 1984). BLAST analysis suggest cross-reactivity with p35 from B. burgdorferi, garinii, and afzelii sources based on 100% homology with the immunizing sequence. Cross-reactivity with p35 from other sources has not been determined.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Recombinant protein corresponding to B. burgdorferi P35, fused to MBP-Tag.
