Outer Surface Protein C, or OspC, is a 22kD immunogenic protein on the outer surface of the spirochete, B.
burgdorferi (Caine, et al., 2017).
OspC’s function is not known, but it is located by lipid-anchoring sites on the outer cell membrane, although it is not believed to be associated with lipid rafts (Grimm, et al., 2004; Toledo, et al., 2014).
OspC has been shown to be a requisite for B.
burgdorferi to establish mammalian infection as well as for spirochaetal transmission from ticks to mammals.
Expression begins during blood feeding by the nymphal tick and continues to be produced in the early stages of infection in the mammal host and is highly immunogenic in mice.
As one of the strategies to evade host humoral responses, spirochetes downregulate OspC production in response to anti-OspC antibodies within 2 to 3 weeks after infection in mice (Grimm, et al., 2004).
OspC also has been proposed to play roles in promoting survival and/or dissemination of spirochetes within the mammalian host.
For example, OspC binds to a tick salivary protein, Salp15, which can protect spirochetes from complement- and antibody-mediated killing (Hovius, et al., 2008).
OspC also binds host plasminogen and this phenotype correlates with invasiveness of spirochetes in mice (Tilly, et al., 2006) and has antiphagocytic properties, aiding spirochete evasion of mononuclear phagocytes during early infection in mammals (Carrasco, et al., 2015)
OspC is required for B.
burgdorferi infection in both immunocompetent and SCID mice (lacking B and T cells) and has been proposed to facilitate evasion of innate immune defences.
An OspC knockout mutant was cleared within the first 48h of infection in a murine host, suggesting a protective role of OspC against innate defences.
Constitutive expression of heterologous lipoproteins in the OspC mutant was shown to restore infection in SCID mice, suggesting that OspC may also play a nonspecific structural role for B.
burgdorferi (Smith, et al., 2018).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes Borrelia burgdorferi OspB, Strain B31 (ATCC 35210), derived by limited dilutional cloning from the original Lyme-disease tick isolate obtained by A. Barbour (Johnson, et al., 1984). BLAST analysis suggest cross-reactivity with p39 from B. burgdorferi, afzelii, and valaisiana sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against B. japonica and americana sources based on 94% homology. Cross-reactivity with OspC from other sources has not been determined.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Recombinant protein corresponding to Borrelia burgdorferi OspC, fused to MBP-Tag.
