Many of the borrelial surface antigens are lipid-modified proteins (i.e.
lipoproteins), although a number of these surface-exposed lipoproteins (OspA, OspB, and OspC) are not found exclusively on the surface of the organism.
These lipoproteins are also detected in the periplasm of the organism and can be shuttled to and from the borrelial surface at different points during infection (Haake, 2000).
The interface between B.
burgdorferi and its human host is its outer surface (and therefore proteins localized to the outer membrane) play an important role in dissemination, virulence, tissue tropism, and immune evasion.
Antibodies directed against outer surface proteins have also been shown to protect animals and humans from infection with B.
burgdorferi (Kenedy, et al., 2012).
OspA (31kD) and OspB (34kD) proteins show a high degree of sequence similarity, are encoded by a 49kb plasmid, share a common promoter and are co-ordinately transcribed (Liang, et al., 2004).
All three Osp proteins (OspA, OspB and OspD) are expressed by B.
burgdorferi residing in the gut of unfed ticks, suggesting that they promote the persistence of the spirochete in ticks between blood meals.
OspB has a contributing role in the adherence of B.
burgdorferi to the tick gut.
The C-terminal of OspB is important for eliciting a protective immune response to OspB.
B.
burgdorferi has the ability to vary its surface proteins in response to immune attack (Neelakanta, et al., 2007).
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:13,000Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：25ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes Borrelia burgdorferi OspB, Strain B31 (ATCC 35210), derived by limited dilutional cloning from the original Lyme-disease tick isolate obtained by A. Barbour (Johnson, et al., 1984). BLAST analysis suggest cross-reactivity with OspB from B. burgdorferi, afzelii, spielmanii, and garinii sources based on 100% homology with the immunizing sequence, and with B. valaisiana based on 99% homology. Crossreactivity with OspB from other sources has not been determined.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Recombinant protein corresponding to B. burgdorferi OspB, fused to MBP-Tag.
