Outer-Surface Protein A (OspA) is a 31kD lipoprotein encoded by Borrelia burgdorferi and is a major component of the spirochete’s extracellular matrix (Stevenson, et al., 1996), probably associated with lipid rafts and serving as a lipid-anchor (Toledo, et al., 2014).
Strain B31 is the type strain (ATCC 35210) for this organism and was derived by limited dilutional cloning from the original Lyme-disease tick isolate obtained by A.
Barbour (Johnson, et al., 1984).
The Borrelia species causing Lyme disease express different OspA serotypes on their surface, B.
burgdorferi (serotype 1), B.
afzelii (serotype 2), B.
garinii (serotypes, 3, 5 and 6) and B.
bavariensis (serotype 4) (Wilske, et al., 1988).
Many of the borrelial surface antigens are lipid-modified proteins (i.e.
lipoproteins), although a number of these surface-exposed lipoproteins (OspA, OspB, and OspC) are not found exclusively on the surface of the organism.
These lipoproteins are also detected in the periplasm of the organism and can be shuttled to and from the borrelial surface at different points during infection.
The interface between B.
burgdorferi and its human host is its outer surface (and therefore proteins localized to the outer membrane) play an important role in dissemination, virulence, tissue tropism, and immune evasion.
Antibodies directed against outer surface proteins have also been shown to protect animals and humans from infection with B.
burgdorferi (Kenedy, et al., 2012).
OspA likely mediates the attachment of B.
burgdorferi to the tick mid-gut by binding the mid-gut receptor TROSPA (Tick Receptor for OspA).
TROSPA is downregulated to allow migration out of the tick mid-gut during feeding, and into the salivary glands before being transmitted to the mammalian host.
This transition is believed to be facilitated by changes in expression of some B.
burgdorferi genes, including OspA (Ding, et al., 2000).
These changes may be regulated by changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH and nutrients) and/or in coordination with the course of infection in the mammal host (Norris, 2006).
A heterodimer of the linked C-terminal half of two OspA serotypes was shown to protect mice from a challenge with spirochetes expressing either OspA serotype 1, 2 or 5, when challenged with infected ticks and in vitro grown spirochetes (Comstedt, et al., 2014).
OspA-specific human Mabs can prevent the transmission of B.
burgdorferi from ticks to mice (Wang, et al., 2016).
More recently, structural analysis of the human antibody LA-2/OspA complex has revealed specific residues that may be exploited to modulate recognition of the protective epitope of OspA potentially offering a new path towards prophylactic passive antibodies (Shivender, et al., 2017).
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes Borrelia burgdorferi OspA, Strain B31 (ATCC 35210), derived by limited dilutional cloning from the original Lyme-disease tick isolate obtained by A. Barbour (Johnson, et al., 1984). BLAST suggest cross-reactivity with OspA from B. burgdorferi and sources based on 100% homology with the immunizing sequence. Crossreactivity with OspA or Osp from other sources has not been determined.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Recombinant protein corresponding to B. burgdorferi OspA, fused to MBP-Tag.
