burgdorferi are believed to be lipoproteins, based on their predicted amino acid sequences.
The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host.
This transition may be facilitated by changes in expression of some B.
burgdorferi genes.
It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host (Stevenson, et al., 1996).
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：25ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes Borrelia burgdorferi Erpd/Arp37, Strain B31 (ATCC 35210), derived by limited dilutional cloning from the original Lyme-disease tick isolate obtained by A. Barbour (Johnson, et al., 1984).
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Recombinant protein corresponding to B. burgdorferi ErpD/Arp37, fused to MBP-Tag.
