burgdorferi differ in their susceptibility to normal human serum and are therefore classified as complement-resistant, complement-sensitive and intermediate complement-sensitive.
Complement resistant bacteria absorb human immune regulators FHL-1/reconectin and factor H using two outer surface Complement Regulator-Acquiring Surface Proteins (CRASP-1 and CRASP-2).
Surface-attached FHL-1/reconectin retains its complement regulatory and cleavage activity (Kraiczy, et al., 2001).
Factor H and FHL-1 serve as cofactors for factor I, a serine protease that cleaves complement component 3b (C3b) directly on the cell surface and thereby ensures resistance of spirochetes to complement-mediated lysis (Kraiczya, et al., 2007).
It is possible that because of discontinuous binding regions in the factor H/FHL-1, a long-distance interaction may be involved in binding of both immune regulators.
Putative coiled-coil structural elements may also be important in the interaction of B.
burgdorferi CRASP-1 with factor H (Kraiczya, et al., 2007).
These proteins represent another immune evasion mechanism of B.
burgdorferi, as bacteria acquire human complement regulators to control complement activation on their surface and prevent formation of toxic activation products (Kraiczy, et al., 2001).
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：25ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes Borrelia burgdorferi CRASP-2, strain B31 (ATCC 35210), derived by limited dilutional cloning from the original Lyme-disease tick isolate obtained by A. Barbour (Johnson, et al., 1984). BLAST analysis suggest reactivity with CRASP-2 from B. burgdorferi sources based on 100% homology with the immunizing sequence. Partialcross-reactivity is expected against B. garinii, B. spielmanii, and valaisianasources based on 91-89% homology. Cross-reactivity with CRASP-2 fromother sources has not been determined.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Recombinant protein corresponding to B. burgdorferi CRASP-2, fused to MBP-Tag.
