CRASP-1 is a 25.9kD multifunctional homodimeric protein with a previously unknown complex protein fold (Cordes, et al., 2005).
CRASP-1 and four additional immune evasion proteins bind combinations of human plasma regulators, including collagen I, collagen III, collagen IV, fibronectin, laminin, factor H-like protein 1 (FHL-1) (resulting in inhibition of complement activation in mammals) and Human Bone Morphogenic Protein 2.
These interactions may contribute to adhesion, bacterial colonization, and organ tropism to promote dissemination of B.
burgdorferi in the host.
Borrelia species contain a large number of both linear and circular plasmids, some of which appear to be repeated or contain fragments of other genes.
These regions may serve as pools of variability for the survival of Borrelia in its multiple environments during its life cycle.
Multiple copies of sequences analogous to CRASP-1 genes have been detected in Borrelia plasmids.
In addition, the sequence for CRASP-1 contains a repeated sequence folded into a stable stem loop structure, typical of RNA genes (Kraiczy, et al., 2004).
CRASP-1 also binds the human terminal complement components C7 and C9 and blocks assembly and membrane insertion of the terminal complement complex (TCC), as confirmed by hemolytic assays, inhibiting polymerization of C9.
When CRASP-1 was expressed ectopically on the surface of serum-sensitive B.
garinii, it blocked TCC assembly and induced serum resistance in the transformed bacteria.
By inducing serum resistance and terminal complement pathway inhibition, B.
burgdorferi is able to survive in the hostile environment of human plasma (Hallström, et al., 2010).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes Borrelia burgdorferi CRASP-1, strain B31 (ATCC 35210), derived by limited dilutional cloning from the original Lyme-disease tick isolate obtained by A. Barbour (Johnson, et al., 1984). BLAST analysis suggests reactivity with CRASP-1 from B. burgdorferi and B. garinii sources based on 100% homology with the immunizing sequence. Partial cross-reactivity is expected against B. spielmanii, afzelii, and valaisiana sources based on 98% homology.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Recombinant protein corresponding to B. burgdorferi CRASP-1, fused to MBP-Tag.
