84-5136-35　［取扱停止］Presenilin 1, CT [KO Validated] 100ul　516561

特徴

• atalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein).
• Requires the presence of the other members of the gamma-secretase complex for protease activity.
• Plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels.
• Stimulates cell-cell adhesion via its interaction with CDH1; this stabilizes the complexes between CDH1 (E-cadherin) and its interaction partners CTNNB1 (beta-catenin), CTNND1 and JUP (gamma-catenin) (PubMed:11953314).
• Under conditions of apoptosis or calcium influx, cleaves CDH1 (PubMed:11953314).
• This promotes the disassembly of the complexes between CDH1 and CTNND1, JUP and CTNNB1, increases the pool of cytoplasmic CTNNB1, and thereby negatively regulates Wnt signaling (PubMed:9738936, PubMed:11953314).
• Required for normal embryonic brain and skeleton development, and for normal angiogenesis (By similarity).
• Mediates the proteolytic cleavage of EphB2/CTF1 into EphB2/CTF2 (PubMed:17428795, PubMed:28269784).
• The holoprotein functions as a calcium-leak channel that allows the passive movement of calcium from endoplasmic reticulum to cytosol and is therefore involved in calcium homeostasis (PubMed:25394380, PubMed:16959576).
• Involved in the regulation of neurite outgrowth (PubMed:15004326, PubMed:20460383).
• Is a regulator of presynaptic facilitation, spike transmission and synaptic vesicles replenishment in a process that depends on gamma-secretase activity.
• It acts through the control of SYT7 presynaptic expression (By similarity).
• Applications:Suitable for use in Immunofluorescence, Indirect Flow Cytometry, Western Blot, and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence: 1:10-1:50Indirect Flow Cytometry: 1:10-1:50Western Blot: 1:1000Immunohistochemistry (paraffin): 1:50-1:100 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.