84-5136-34　［取扱停止］LAMP2 [KO Validated] 100ul　516559

特徴

• Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live.
• Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation.
• Plays a role in lysosomal protein degradation in response to starvation (By similarity).
• Required for the fusion of autophagosomes with lysosomes during autophagy (PubMed:27628032).
• Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes (PubMed:27628032).
• Required for normal degradation of the contents of autophagosomes (PubMed:27628032).
• Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits (PubMed:20518820).
• Is not required for efficient MHCII-mediated presentation of endogenous antigens (PubMed:20518820).
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:8000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.