84-5136-27　SCT (Secretin) Glycerol-free 100ug　364999-X

特徴

• Secretin is a hormone that controls the secretions into the duodenum, and also separately, water homeostasis throughout the body.
• Acting as an anti-Mallerian hormone it is produced in the S cells of the duodenum in the crypts of Lieberkahn.
• Its effect is to regulate the pH of the duodenal contents via the control of gastric acid secretion and buffering with bicarbonate.
• It is notable for being the first hormone to be identified.
• In humans, the secretin peptide is encoded by the SCT gene.
• It has recently been discovered to play a role in osmoregulation in the hypothalamus, pituitary, and kidney.
• Secretin is a linear peptide hormone, which is composed of 27aa and has a molecular weight of 3055.
• A helix is formed in the amino acids between positions 5 and 13.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunohistochemistry (Paraffin): 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control Included:364999A: SCT, Positive ControlSuppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 50mM DTT, 0.02% BPB, 0.02% sodium azide, 50% glycerol)
• DAB: 10ul/wellECL: 5ul/wellStore at -20°C.
• Stable for 6 months after receipt.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.